Optimization and Purification of Lipase through Solid State Fermentation by Bacillus Cereus Msu as Isolated from the Gut of a Marine Fish Sardinella Longiceps

A lipase producing bacterium, Bacillus cereus MSU AS was isolated from the gut of a marine fish Sardinella longiceps. In total, seven agricultural waste substrates (coconut oil cake, neem oil cake, onion skin waste, wheat bran, jack fruit waste, banana skin peels and pomegranate skin peel) were selected for solid state production of lipase by B. cereus MSU AS. Among the tested substrates, onion skin waste supplemented medium was found to be yielded maximum lipase and hence it was selected as a major substrate for further study. On optimization, 10% moisture content, 0.5% maltose (carbon source), 0.5% ammonium hydrogen carbonate (nitrogen source), 0.4% gingilly oil (triglyceride) and 9% NaCl concentration were favoured more on production of lipase by B. cereus MSU AS. Further the lipase produced by the candidate strain was purified and its molecular weight was determined as 49 kDa by SDS-PAGE analysis.